Leica HyVolution SP8 Confocal Microscope
This system consists of an SP8 confocal scan head mounted on a Leica DMi8 inverted microscope. For excitation, the following lasers are available: 405 nm diode, Argon (458 nm, 476 nm, 488 nm, 496 nm, and 514 nm), 594 nm HeNe, 561 nm diode-pumped solid state, and a 633 nm HeNe. For detection, this system possess three internal Hybrid Detectors (GaAsP), two internal PMT detectors, and a transmitted light PMT. All objectives are plan apochromatic and corrected for confocal imaging.
Objectives include: 10x/0.40, 20x/0.75 (multi-immersion), 40x/1.30 (oil immersion), 40x/1.10 (water immersion with MottCorr motorized correction collar), and 63x/1.40 (oil immersion). Additionally, the system is equipped with 8 kHz resonant (high speed) and galvo (high pixel density) scanners; a zGalvo stage for precision z-sectioning; a Tokai Hit stage top incubator for live cell imaging; AOBS (acoustic-optical beam splitter) and sequential scanning capabilities. FRAP, FRET, time-lapse imaging, co-localization analysis, and 3D rendering are all available through the LAS X software. Additionally, the HyVolution 2 software couples the instrument’s acquisition parameters to SVI Huygens software allowing the option to integrate xyz deconvolution directly into the imaging workflow.
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Leica TCS SP8 gated STED 3x Confocal Microscope
A gated STED super-resolution confocal laser scanner is mounted on an inverted DMI6000 microscope equipped with a motorized stage. The system is equipped with the following lasers: 405 nm, Argon (458, 476, 488, 496, 514 nm), a tunable white light laser (470-670 nm), and the STED laser (592 nm and 775 nm). The system has both PMTs and hybrid (GaAsP) detectors for higher sensitivity detection, greater dynamic range and low light imaging capabilities. Additionally, the system is equipped with resonant (high speed) and galvo (high pixel density) scanners; a zGalvo stage for precision z-sectioning; an environmental chamber for live cell imaging; AOBS (acoustic-optical beam splitter) and sequential scanning capabilities. The system is capable of 3D rendering, FRAP, FRET, time-lapse, and Huygens deconvolution. The objectives possess superior color-correction and are optimized for confocal applications. These objectives include a plan apochromatic 10x/0.4, 20x/0.75 (multi-immersion), 40x/1.1 (water immersion) 40x/1.3 (oil), 63x/1.4 (oil), and 100x/1.4 (oil). The system achieves a lateral resolution of up to 50 nm and an axial resolution of approximately 130 nm, depending on the sample preparation and fluorophore properties. These capabilities make it possible to visualize nanoscale details of organelles, protein complexes, and other structures in their native 3D context. With features like gated detection to enhance signal-to-noise ratio and multicolor imaging capabilities, the SP8 Gated STED 3X is ideal for applications in cell biology, neuroscience, and materials science. Its modular design and intuitive software further streamline workflows, enabling researchers to push the boundaries of optical imaging.
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Olympus SPIN SR-10 Spinning Disc Confocal Microscope
Olympus SpinSR microscope coupled with Yokogawa CSU-W1 Spinning Disk:
- Provides high speed confocal image acquisition with an ultrawide field of view that is 4X the size of conventional spinning disks
- Super-resolution can be performed using Olympus super-resolution IXplore SpinSR (SoRa Lens) system for live cell super resolution imaging on a single sample with IXplore motorized magnification changer
- Ideal for membrane trafficking imaging, intracellular trafficking, photo-stimulation/deactivation, molecular dynamics, imaging of fast calcium events (sparks, waves, puffs), time-lapsed imaging, photo-bleaching, photo-switching.