3i Cleared Tissue LightSheet Microscope (CTLS)
The Cleared Tissue LightSheet (CTLS) is a large field light-sheet microscope designed for high-speed imaging of whole organs that have been optically cleared. The CTLS creates a focused sheet with a narrow waist for improved optical sectioning. The waist of the sheet is rapidly shifted along the axis of propagation using a spatial light modulator (SLM). In combination with a macro zoom microscope using high NA large field of view lenses, CTLS can image large fields with high resolution in a short time.
Our 3i CTLS has several key technical features:
Objectives: 1.5X/0.37 NA (default); 1.0X/0.25 NA (for larger samples).
Camera: ORCA-fusionBT C15440.
Laser lines: 488, 561, 642 nm are available.
Emission filter: 432/515/595/681/809 nm Penta-Band Bandpass.
Resolution: The CTLS can image a volume of 1 cm³ at up to 1µm x 1µm x 3µm (XYZ) resolution.
Default pixel size: 1µ[email protected], 0.5µ[email protected]. Special sampling requirements should be discussed with the core manager and director. Read More