School of Medicine Light Microscopy Imaging Core

Lattice LightSheet Microscope (LLS)

3i Lattice LightSheet Microscope (LLS)

The Lattice LightSheet Microscope (LLSM) highlights two significant advantages: faster imaging and significantly reduced photo-bleaching and photo-damage. Originally pioneered by the esteemed 2014 Nobel Laureate, Dr. Eric Betzig, this cutting-edge technology is now readily available through the company 3i. LLSM is Ideal for visualizing intracellular dynamics in live cells in 3D, with incredibly low photobleaching and photodamage. Additionally, the SlideBook software provides users with easy acquisition, data processing, and visualization.

Our 3i LLSM is equipped with following features:

Laser lines: 488, 561, and 638 nm

Emission filter: 446/523/600/677 nm Quad-Band Bandpass

Objectives: excitation: 28x 0.7 NA water dipping lens, 3.7 mm working distance; Detection: Nikon 25x 1.1 NA water dipping lens, 2.0 mm working distance, the final magnification is 62.5x magnification.

Environmental control: From RT to 42 Celcius, no CO2

Camera: Hamamatsu ORCA Fusion BT sCMOS

Field of View: Read More

Resources:

Mitosis (3i website)
Hematology (3i website)
Multicellular (3i website)
Hela cells (3i website)
Cytoskeleton (3i website)
Cell Systems (3i website)
Neuroscience (3i website)
Model Organism (3i website)
Photo Manipulation (3i website)
Plant Biology (3i website)
Video courtesy of The University of Queensland, Australia