Empowering Science, Enabling Discovery: Unveiling the Invisible with Our Imaging-Based Platforms
Lattice LightSheet Microscope (LLS)
3i Lattice LightSheet Microscope (LLS)
The Lattice LightSheet Microscope (LLSM) highlights two significant advantages: faster imaging and significantly reduced photo-bleaching and photo-damage. Originally pioneered by the esteemed 2014 Nobel Laureate, Dr. Eric Betzig, this cutting-edge technology is now readily available through the company 3i. LLSM is Ideal for visualizing intracellular dynamics in live cells in 3D, with incredibly low photobleaching and photodamage. Additionally, the SlideBook software provides users with easy acquisition, data processing, and visualization.
Objectives: excitation: 28x 0.7 NA water dipping lens, 3.7 mm working distance; Detection: Nikon 25x 1.1 NA water dipping lens, 2.0 mm working distance, the final magnification is 62.5x magnification.
Environmental control: From RT to 42 Celcius, no CO2
Camera: Hamamatsu ORCA Fusion BT sCMOS
Field of View:
Field Of View (FOV): FOV Size
X (µm)
Y (µm)
Z (µm)
2048 x 2048 (pixels)
200
200
40 to 50
1024 x 1024 (pixels)
100
100
40 to 50
512 x 512 (pixels)
50
50
40 to 50
Resolution:
Highest achievable lateral and axial resolution for gaussian fit PSF’s using the 0.55 x 0.493 mask NA follow:
Wavelength (nm)
X (nm)
Y (nm)
Z (nm)
488
375
375
775
560
415
415
835
642
485
485
940
For SIM, the axial resolution is ~1.5x or better, depending on which patterns you are using. Common target numbers would be as follows:
Wavelength (nm)
Z (nm)
488
~500 nm
560
~560 nm
642
~630 nm
Speed of system: Maximum frame rate is 333 fps at 512×512
